Abstract 4202: Resection of primary osteosarcoma tumors terminates self-seeding, increasing lung metastasis

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Abstract Like many solid tumors, osteosarcoma (OS) kills patients through metastatic spread. In the case of OS, metastasis occurs almost exclusively to lung. Metastases most often arise after both resection of a primary tumor and completion of chemotherapy. In seeking to understand this long-appreciated pattern of spread, we sought to explore whether the presence of a primary tumor protects mice from developing OS lung metastasis through the process of self-seeding, and whether resection then faciliates metastatic lung colonization. Our data show that resection of a primary orthotopic tumor triggers the spread of OS tumor cells into the lung. Mice bearing a primary tumor have fewer labeled tumor cells recovered in the lung after IV inoculation. Many labeled cells can be recovered in the primary tumor. Supernatants from OS cell cultures drive OS cell chemotaxis with greater potency than serum. Several specific soluble factors produced by OS cells also drive chemotaxis of those same cells. Disruption of these signals may prevent self-seeding. Manipulation of this system may be used to remove OS cells from circulation. Citation Format: Ryan D. Roberts, Amanda J. Saraf, Helene Le Pommellet, Randall Evans, Amy C. Gross. Resection of primary osteosarcoma tumors terminates self-seeding, increasing lung metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4202.