Abstract 3349: In-silico analysis identifies the unique role of the ADRB2-USP20 axis in T cell lymphoma

Abstract

Abstract T cell lymphoma represents an aggressive subgroup of lymphoma having a poor prognosis. Many factors, including psychological stress, define the onset/progression of the disease. Although psychological stress can cause several health problems, preclinical and clinical reports regarding its role in cancer have been mixed. Therefore, in this study, we sought to determine the role of psychological stress hormone in the progression of T cell lymphoma and provide a mechanistic framework of activity. Initially, we used the cBioportal and performed expression analysis of genes involved in stress pathways with large-scale cancer genomics TCGA datasets having nearly 10071 patient samples. Through a series of in-silico investigations, we identified Adrenoceptor Beta 2 (ADRB2), a key mediator of stress, highly expressed in T cell lymphoma compared to other tumors.Furthermore, the expression of ADRB2 was not gender-specific and equally represented in both males and females. String analysis further identified the interaction of ADRB2 with Ubiquitin carboxyl-terminal hydrolase 20 (USP20), which directly regulates Hypoxia-inducible factor 1-alpha (HIF1-A) and ADRB2. This regulation could lead to enhanced glucose metabolism to support T cell lymphoma growth and progression. Collectively, our in-silico findings suggest that psychological stress releases hormones that could modulate T cell lymphoma proliferation through the ADRB2-USP20 axis. Ongoing preclinical studies with stress hormones will be presented to provide undescribed mechanisms of their protumorigenic activity through the ADRB2-USP20 axis and their role in regulating cancer-specific metabolism. We sought to determine the role of psychological stress hormone These studies will provide an opportunity to learn the involvement of stress hormones in cancer progression and provide a framework for developing a novel approach for stress management during cancer. Citation Format: Rajan Kumar Tiwari, Ajay Kumar. In-silico analysis identifies the unique role of the ADRB2-USP20 axis in T cell lymphoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3349.