Abstract 3060: The role of miR-135b in clinical outcome and metastasis in colorectal cancer

Abstract

Abstract Colorectal cancer (CRC) is one of the leading causes of cancer death in both the Western world and Taiwan. Age, gender, and genes are important risk factors for colorectal cancer. Activation of Wnt signaling pathway is an important step involved in the pathogenesis of colorectal cancer. Approximately 70-80% of sporadic colorectal carcinomas have somatic mutations that inactivate APC. But, only 30% CRC patients in Taiwan have APC gene mutation that was significantly lower than the other countries. Thus, the other mechanism involved in APC gene inactivation may contributed to CRC progression in Taiwan. Here, we focus on the Wnt signaling associate microRNAs. Four wnt signaling-associated microRNAs were selected in this study, including miR-21, miR-200a, miR-135a and miR-135b. A total of 156 colorectal cancer patients were enrolled in this study. The expression of miR-21, miR-200a, miR-135a, miR-135b and wnt signaling association genes were analyzed by quantitative RT-PCR and immunohistochemistry. The expression levels of miR-21, miR-200a and miR-135a were not correlated with clinical parameters of CRC patients. The expression of miR-135b were positive correlated with gender, tumor stage, and lymph node metastasis. No any significantly correlation were found between miR-135b, Wnt, β-catenin and APC. But the expression levels of miR-135b were positive correlated with the upstream gene STAT3, β-catenin and C-Myc. Thus, we suggested that upregulation of miR-135b may through β-catenin mediated STAT3 activation. Citation Format: Wei-Ran Wang, Yawen Cheng, Nan-Yung Hsu Hsu. The role of miR-135b in clinical outcome and metastasis in colorectal cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3060. doi:10.1158/1538-7445.AM2015-3060