台灣大腸直腸癌患者之Ｗnt/β-catenin訊息路徑相關基因與microRNA表現之相關性

Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer death in both the Western world and Taiwan. Age, gender, and genes are important risk factors for colorectal cancer. Activation of Wnt signaling pathway is an important step involved in the pathogenesis of colorectal cancer. Approximately 70–80% of sporadic colorectal carcinomas have somatic mutations that inactivate APC. But, only 30% CRC patients in Taiwan have APC gene mutation that was significantly lower than the other countries. Thus, the other mechanism involved in APC gene inactivation may contributed to CRC progression in Taiwan. Here, we focus on the Wnt signaling associate microRNAs. We selected four wnt signaling associated microRNAs, including miR-21, miR-200a, miR-135a and miR-135b. A total of 156 colorectal cancer patients were enrolled in this study. The expression of miR-21, miR-200a, miR-135a, miR-135b and wnt signaling association genes were analyzed by quantitative RT-PCR. The results showed that expression of miR-21, miR-200a and miR-135a were not associated with clinical parameters. Expression of miR-135b were positive correlated with gender, tumor stage, and lymph node metastasis. No any significantly correlation were found between miR-135b, Wnt,β-catenin and APC. But we can observed the positive correlation between STAT3, the upstream gene of miR-135b, β-catenin and C-Myc. Thus, we hypothesized that upregulation of miR-135b may throughβ-catenin mediated STAT3 activation