Abstract
Abstract This study compares quantitative microRNA expression profiles of normal endometrial tissue and the three primary endometrial tumor types; endometrioid adenocarcinoma, papillary serous adenocarcinoma, and carcinosarcoma, representing a spectrum of tissue involvement, aggressiveness, and prognosis/ recurrence risk. Methods: Total RNA was prepared directly from primary tissues and microRNA expression was profiled in two ways. Expression levels of 667 human microRNAs were assessed by qPCR using TaqMan microRNA Arrays (Applied Biosystems). In addition, a size-selected small RNA cloning strategy developed in our lab was employed to search for additional microRNAs via deep sequencing on the Illumina/Solexa platform. Results: A number of recent surveys have implicated a small set of candidate microRNAs in endometrial cancers. Among theses are miR-103, miR-107, miR-141, miR-210, as well as the miR-200 family, particularly miR-200c. Our results confirm these previous studies as well as implicating several additional microRNAs. Conclusions: Quantitative microRNA expression profiling of endometrial cancers demonstrates differential expression that segregates with tumor type and clinical prognosis. In addition, a number of specific microRNAs appear to be consistently either over- or under-expressed in these cancers compared to normal endometrium. It is these microRNAs, such as miR-210 and miR-200c, that comprise a set of markers for quantitative validation and target ascertainment with the potential to provide important correlates to cancer type and prognosis/ recurrence risk. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3040.
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