Abstract
Abstract InIntroduction: microRNA (miRNA) are small non-coding RNAs which target specific mRNA. Recent progression of RNA extraction technology from formalin-fixed paraffin-embedded (FFPE) tissues enables us to access miRNA profiling using world wide stored specimens. Here, we present miRNA profiling of gastric cancer patients from FFPE specimens. Method: First, we examined the level of cross-linking caused by formalin and degradation of mRNA and miRNA from day to year levels. Then, we established the criteria of evaluable RNA from FFPE specimens. The miRNA profiling was obtained from 47 gastric cancer patients who received operations from 1997 to 2007 using 3D-Gene human miRNA chip (Toray) which contains 885 miRNA species. Results: Out of 47 paired samples, 37 pairs (78.8%) were determined evaluable with our criteria. As results, we showed that 30 miRNAs were significantly down-regulated and that 15 miRNAs were up-regulated in gastric cancer in comparison to normal gastric tissue. Of these, 18 miRNAs, including mir-21 and mir-34a, were identified as a prognostic factor of gastric cancer patients. Furthermore, we found 25 miRNAs have association with the recurrence of adjuvant oral Tegaful treatment and 5 miRNAs have association with lymph node metastasis. Conclusion: We have discovered miRNAs that are associated with prognosis, chemosensitivity and lymph node metastasis in gastric cancer patients. miRNA profiling using FFPE specimens is a useful and promising method of evaluation of world wide stored specimens that accompany may valuable clinical data. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3039.
Published Version
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