Abstract 2784: Association between polymorphisms in angiogenesis-related genes and risk of urothelial carcinoma in Taiwan

Abstract

Abstract Introduction: Urothelial carcinoma (UC) including cancers of bladder, renal pelvis and ureter. Tumor staging and grading are considered to be useful indices for cancer prognosis. Recently, more and more studies indicated that angiogenesis is a necessary step in tumor growth and metastasis. This study aimed to investigate the relationship between single nucleotide polymorphisms (SNPs) of four angiogenesis-related genes including FIH-1, VHL, HIF-1 and VEGF and clinico-pathological characteristics, recurrence, and survival rate of UC. Materials and Methods: In this case-control study, a total of 850 pathologically- confirmed UC cases and 850 cancer-free controls, frequency-matched on age, were recruited from those who admitted to the same hospitals for a health examination. Genotyping is determined using PCR-RFLP and Real-time PCR. Written informed consent was obtained from each of participants. This protocol was approved by the institutional review boards of the collaborated hospitals. Results: We found that study subjects with cigarette smoking, alcohol consumption, occupational exposure, arsenic exposure, and high-salt diet have a significantly higher UC risk of 2.0, 1.6, 1.8, 1.9 and 1.5. Study subjects carrying C/G and G/G genotypes of FIH-1 gene had a 1.3-fold risk of developing to stage II or worse status. Cigarette smokers with the C/C genotype of VHL gene had a higher UC risk of 2.4. In addition, cigarette smokers with the T/T genotype of VEGF-460 C/T polymorphism had 1.6- fold UC risk of developing to stage II or worse status. In the combined analysis, study subjects who carried 2 or more risk genotypes of angiogenesis-related genes had a significantly higher UC risk of 1.6 for developing to T2 and stage II or more serious. Conclusions: Our results suggested a significant association between polymorphisms in angiogenesis-related genes and morbidity and tumor stage of UC, however, without a significant association between these SNPs and tumor grade. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2784. doi:10.1158/1538-7445.AM2011-2784