Abstract 2366: Transcriptome analysis of human metastasis prostate cancer cells deciphers new function of Esrrb

Abstract

Abstract Orphan nuclear receptor family member, Estrogen Related Receptor β (esrrb) became a research hot topic in recent years. Although the function of esrrb is relatively well studied in stem cells and early embryonic development, little is known about its function in cancer. Esrrb is reported to arrest prostate cancer cell cell-cycle by upregulating p21. Here we investigate the transcriptome alteration induced by esrrb expression and its synthetic ligand DY131 in a human prostate cancer cell line. We distinguished 205 qualified differentially expressed (DE) genes regulated by esrrb alone, and 3202 genes with DY131 treatment with esrrb expression, indicating esrrb has both ligand independent and ligand dependent activity. From the esrrb-regulated transcriptome, we established a hypothetical esrrb transcription regulation model in prostate cancer cells by matching the DNA motif recognized by transcriptional factors (TFs) to the promoter sequences of differentially expressed genes. We found esrrb-regulated gene expression is mediated by TAGLN, HOXB2 and HOXB8 from our model. Our findings potentially provide multiple markers to characterize esrrb's function in prostate cancer. Citation Format: Yuan Lu, Jilong Li, Jianlin Cheng, Dennis B. Lubahn. Transcriptome analysis of human metastasis prostate cancer cells deciphers new function of Esrrb. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2366. doi:10.1158/1538-7445.AM2014-2366