Abstract 1985: Vitronectin expression in primary lung cancers

Abstract

Abstract Rationale Vitronectin (Vn) plays a role in extracellular matrix (ECM) remodeling during tumorigenesis. Vn is present in human bronchial submucosal glands and it is secreted by A549 lung adenocarcinoma cell line. However, whether Vn is differentially expressed in tissues of primary lung cancer has not been explored. Methods Lung tissue from 22 primary lung carcinoma and 36 non-lung cancer subjects were obtained from fibrobronchoscopy. Sections were analyzed by histo- immunohistochemical methods. The total area occupied by ECM, surface and gland epithelium, as well as the percentage area occupied by Vn at each of these localizations were measured. Chi-square, t-student, U-Mann Whitney and ANOVA tests were used in group comparisons. Statistical significance was tested at P<0.05. Results Vn expression was observed in bronchial glandular and surface epithelium, as well as in ECM . In total, the average area of ECM, surface or glandular epithelium was 0.289mm2 (± 0.032), 0.043mm2 (±0.009), 0.084mm2 (±0.031), respectively. The percentage area occupied by Vn in ECM, surface or glandular epithelium, was 4.899 (±1.186), 7.279 (±1.623), 3.285 (±1.038), respectively; with not significant statistical differences between lung cancer and non-cancer subjects or within different types of primary lung cancer (Table 1). Conclusion There were no statistically significant differences in Vn expression between lung cancer and non-cancer subjects, or within primary lung carcinomas. Demographic characteristics and measurement comparisons of subjects studiedConditionNon-cancer(n=36)Cancer(n=22)PAge (Years)59.810(+/- 13.556)65.790(+/- 10.648)0.074Sex*0.752Male18 (50.0%)11 (50.0%)Female18 (50.0%)11 (50.0%)ECM area (mm2)0.311(+/- 0.204)0.424(+/- 0.246)0.059Area occupied by Vn in ECM (percentage)2.894(+/- 2.717)3.584(+/- 3.220)0.384Surface epithelium area (mm2)0.221(+/- 0.678)0.052(+/- 0.023)0.398Area occupied by Vn in surface epithelium (percentage)5.583(+/- 3.939)6.478(+/- 7.757)0.662Gland epithelium area (mm2)0.142(+/- 0.129)0.086(+/- 0.079)0.389Area occupied by Vn in glandular epithelium (percentage)4.151(+/- 2.810)1.424(+/- 0.505)0.200 Note: This abstract was not presented at the meeting. Citation Format: Lina M. Salazar-Pelaez, Ivan Martinez-Forero. Vitronectin expression in primary lung cancers. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1985. doi:10.1158/1538-7445.AM2014-1985