Abstract 1925: Effect of Averrhoa carambola extract on the antineoplastic and apoptotic activities in mice

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Abstract Medicinal plants play significant roles in the treatment of different diseases. Averrhoa carambola has important clinical values in the treatment of cancer. However, its antineoplastic and apoptotic activities are still obscure. In this study, we were aimed to investigate those potentials of Averrhoa carambola in mice. For this, first the powder, prepared from the leaf and flower of Averrhoa carambola, were suspended with methanol to yield methanol extract of leaf and flower. Antineoplastic effect of leaf (25 and 50 mg/kg) against Ehrlich Ascites Carcinoma (EAC) was assessed by evaluating the viable tumor cell count, survival time, body weight and hematological parameters of EAC cell bearing mice. The result showed that the treatment with leaf significantly (p<0.05) decreased viable EAC cells and body weight gain thereby increasing the life span of EAC bearing mice and also revive the altered hematological (Hb, total RBC and WBC) parameters compare to those with normal values. Fluorescence microscopic view of 4,6-diamino-2-phenylindole (DAPI) staining demonstrated that leaf induced the alterations (i.e., nuclear condensation, fragmentation, membrane blabbing and apoptosis of cells) in the morphology of EAC cells. And, the quantitative analysis of p53 mRNA level by RT-PCR showed that p53 gene expression in leaf treated EAC cell was upregulated in respect to untreated control. These findings indicate that the induction of apoptosis in leaf-treated EAC cell was probably mediated through p53-dependent pathway. From this study, it can be concluded that the leaf of Averrhoa carambola exhibited potent antineoplastic and apoptotic activities that might be a potential agent for prevention of cancer in mice. Citation Format: Md. Zahid Hasan, Ayesha Siddika. Effect of Averrhoa carambola extract on the antineoplastic and apoptotic activities in mice [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 1925.