Abstract
Abstract We aimed at generating a clinically meaningful in vivo platform for preclinical drug screening in colorectal cancer. Surgical specimens were collected from 143 patients, and 97 carcinomas were engrafted in immunodeficient mouse (tumor take rate 67%). To develop in vivo platform suitable for drug screening, 39 of xenografted carcinomas were selected and expanded satisfactorily in mice with a mean time to reach a size of 1000-1500 mm3 of 90 ± 20 days. In order to validate the similarity of molecular characters between the patient tumor and carcinomas obtained from xenografts, we conducted the mutation status, an expression array, arrayCGH, and other molecular profiling in colon cancer to look at the molecular progression and transition along with primary tumor, metastatic tumor and xenograft model. Histological analysis and the molecular profiles of tumors revealed a high degree of similarity between the patient tumor and xenografted carcinomas. The xenografted tumors maintain their genotypic features of original tumors, suggest that the in vivo platform can be useful for the personalized drug development in colorectal cancers. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1315. doi:1538-7445.AM2012-1315
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