Abstract 10782: Optimization of Preservation Solution and Temperature of Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Patch for Regenerative Therapy

Abstract

Background: Regenerative therapy for heart failure is being practical as a new treatment. Since cell products contain living cells, it is important to apply the storage method according to the characteristics of the product in order to maintain its structure and function. A lot of studies have been done on suitable storage solutions for organs, and organ storage solutions may also be effective for cell product preservation, especially tissues with three-dimensional structures. In this study, we investigated the optimal storage conditions for human induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) patches. Methods and Results: We evaluated the properties of iPSC-CM patches after storage in cell culture buffer solution (BS), intracellular fluid type organ preservation solution A (PSA), and extracellular fluid type organ preservation solution B (PSB) and under room temperature or 4°C. Although the patches maintained their sheet-like morphology up to 120hrs , the sarcomere structure was disrupted, and the viability decreased over time after 48hrs of storage in BS at room temperature. Myocardial contraction/relaxation velocity and cytokine production after reculture of the stored patches showed a significant decrease after 96hrs of storage in BS at room temperature. We tested whether PSA and PSB improve the preservation of the patches compared to the BS in 48hrs storage. As a result, contraction velocity after reculture was comparable for PSA (17.1±4.4 μm/sec), PSB (10.0±0.9 μm/sec) and BS (12.9±1.4μm/sec). However, cell viability after storage was significantly decreased in PSA (13.9±19.9%) and PSB (68.4±26.1%) compared to BS (90.6±4.1%). Although sarcomere structure was maintained in BS, it was disrupted in PSA and PSB. No improvement was observed in contraction velocity, cell viability and sarcomere structure in PSA at 4°C compared to BS at room temperature. Conclusions: The iPSC-CM patches maintained their structure and function for up to 48 hrs under storage conditions in BS at room temperature. The buffer solution, intracellular or extracellular fluid type organ preservation solution have different ionic balances, suggesting that the optimal storage conditions differ according to tissue structure and cell type.