Abstract 1055: Character of tumor assocated protien recognized by monoclonal antibody against yunnan gejiu lung cancer

Abstract

Abstract Character of tumor assocated protien recognized by monoclonal antibody against Yunnan Gejiu lung cancer: about protein N35 WANG Qinqin The Clinical Reseach Center. The First Hospital of Kunming Medial College Kunming, Yunnan, P.R. China 650032 ABSTRACT Objectives: To identify and characterize lung cancer associated protein N35 and attempt to learn the prospective possibility of the clinical application of the protein N35. Methods: Immunoprecipitation, immunoblotting, differential centrifigation and subcellular assay, immunohistochemistry, Nglycanase digestion, mitotic cell immunoflourescence and multiple methods of affinity chromatography have been used with the monoclonal antibody N-35 to detect the distribution of the protein N35 among the various cancer cell lines and normal human tissue, the relationship between the protein N35 and glycoprotein, the location of the subcellular structure and chromosomal domain of the protein N35,the most effective way of purification of tumor associated protein N35. Results: The protein N35 is a glycoprotein, distributes to the human lung cancer cell line GLC-82, human cervical cancer cell line Hela, human hepatic cancer cell line HepG-2 and human breast cancer cell line PMC with different relative molecular mass(Mr), but no expression of the protein ingredient in normal human fresh tissue; concentrates at the nuclei significantly, much more than at the mitochondrail and membrane, locates at the centriole of the chromosomal domain. Conclusions: The lung cancer associated protein N35 might be expressed only by the cancer cells and related with the proliferation of cancer cells as a role of tumor cell growth regulator. Keywords: Lung cancer cells, Tumor associated protein, Glycoprotein, Centriole Qinqin Wang Prof of The Clinical Reseach Center. The First Hospital of Kunming Medial College 295 Xichang Rd. 650032 Kunming Yunnan, P.R. China Tel:868714191985 Fax: 868715336015 Email: zys@public.km.yn.cn Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1055.