Abstract
The absorbance produced by a given weight of corti-costeroid in the blue tetrazolium method is shown to be a function of the apparent pH as measured by a glass and saturated calomel electrode. In general, the absorbance decreases as the apparent pH decreases, irrespective of the cause of the apparent pH change. An equation is derived which relates the absorbance to the apparent pH for hydrocortisone solutions, and the relationship is shown to be similar for several representative corticosteroids. It is recommended that the apparent pH be determined on all samples that are run without a prior acidic or basic extraction to determine if low readings are due to interference or to lower than expected quantities.
Published Version
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