Abstract
Pueraria lobata (Willd.) Ohwi. is a widely used medicinal plant in Korea, China, and Japan. The flower of P. lobata (Puerariae Flos) contains various bioactive substances such as triterpenoidal saponins and isoflavonoids. In this study, we developed a quantitative analysis of the isoflavones of Puerariae Flos by quantitative proton nuclear magnetic resonance (qHNMR) spectroscopy using the internal calibrant (IC). From the qHNMR results, the isoflavone content was found to be 7.99% and 10.57% for the MeOH sonication extract (PLs) and the MeOH reflux extract (PLr) of Puerariae Flos, respectively. The quantified isoflavone content was validated using the conventional analytical method, high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The present study shows that validated qHNMR spectroscopy is a reliable method for quantifying and standardizing the isoflavone content in Puerariae Flos.
Highlights
Pueraria lobata (Willd.) Ohwi. is a perennial plant belonging to the Leguminosae family [1]
Isoflavonoids are a large group of secondary metabolites in plants and include isoflavanones, isoflavans, rotenoids, and pterocarpans, which consist of two benzene rings linked by a three-carbon bridge that produces a 3-phenylchromen-4-one backbone [14,15]
The quantitative H NMR (qHNMR) method was developed and successfully applied as a simple, rapid, sensitive, and reliable tool to quantify all seven isoflavones in the Puerariae Flos extracts obtained by two different methods
Summary
Pueraria lobata (Willd.) Ohwi. is a perennial plant belonging to the Leguminosae family [1]. The high medicinal and nutritional value of the root of P. lobata (Puerariae Radix) has proven to be responsible for its traditional use as an ingredient in soups and Chinese cuisines [2,3]. There has been scientific interest in the flowers of P. lobata (Puerariae Flos) owing to the presence of isoflavonoids as the major constituents [1,9,10]. In addition to the roots and flowers, isoflavonoids were reported from different parts of P. lobata such as outer barks [11], stems [12], and leaves [13]. Isoflavonoids are a large group of secondary metabolites in plants and include isoflavanones, isoflavans, rotenoids, and pterocarpans, which consist of two benzene rings linked by a three-carbon bridge that produces a 3-phenylchromen-4-one backbone [14,15]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
