Abstract
Absolute digital(d)PCR quantification of micro(mi)RNA molecules for the diagnostic screening of colon cancer in human stool: A methodological review
Highlights
Determining the integrity of RNA starting materials is a critical step in gene expression analysis
Researchers have used the ribosomal ratio in both slab gel analysis and as a feature within the Bioanalyzer software to characterize the state of RNA intactness
Development of the RNA Integrity Number (RIN) tool The RIN software algorithm was developed for samples acquired with the Eukaryote Total RNA Nano assay on the Agilent 2100 Bioanalyzer System
Summary
Determining the integrity of RNA starting materials is a critical step in gene expression analysis. The Bioanalyzer software generates an electropherogram and gel-like image and displays results such as sample concentration and the so-called ribosomal ratio. The electropherogram provides a detailed visual assessment of the quality of an RNA sample.
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