Absence of Expression of OCT-2 in Gastric Cancer Cells

José Paulo Freire,José Crespo Mendes De Almeida,Maria Emilia Oliveira,Ana Rute Fernandes,Henrique Bicha Castelo,Maria Conceição Crujo

doi:10.4236/ajmb.2014.43014

José Paulo Freire, José Crespo Mendes De Almeida + Show 4 more

Open Access

https://doi.org/10.4236/ajmb.2014.43014

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Abstract

OCT-2, a member of the POU homeodomain family of transcription factors, has been implicated in gastric cancer lymph node metastasis on a single publication so far. Other members of the same family of transcription factors were found to have a role in intestinal metaplasia and gastric cancer (OCT-1) and in trophectoderm differentiation of embryonic stem cells (OCT3/4) via modulation ofCDX2. These findings prompted us to evaluate whether OCT-2 could in fact have a role in gastric oncogenesis and lymph node metastasis in human gastric cancer, designing an immunohistochemistry study of the putative expression of OCT-2 on human gastric cancer tissue of 69 surgical archival specimens of intestinal and diffuse type. We could not find expression of OCT-2 on any of our cancer tissues, metaplasia or normal gastric mucosa despite the expression of OCT-2 on tonsil lymphatic cells (tissue test) and normal lymph nodes. We concluded that, based on immunohistochemistry, OCT-2 does not have a role in gastric oncogenesis nor does have any relationship with lymph node metastasis in gastric cancer.

Highlights

OCT-2 is a member of the POU homeodomain family of transcription factors [1] and was originally identified as a factor which was expressed only in B Lymphocytes
It was demonstrated that OCT-1, another member of the POU homeodomain family of transcription factors that share with OCT-2, the ability to recognize the canonical octamer motif (ATGCAAAT) that regulates the transcription of various genes, is over expressed in intestinal metaplasia and intestinal type carcinomas in human gastric cancer [4]
Lymph nodes near the carcinoma tissue stained positive for OCT-2 but no staining was present in cancer tissue, metaplasia or normal gastric mucosa (Figure 3)

Summary

Introduction

OCT-2 is a member of the POU homeodomain family of transcription factors [1] and was originally identified as a factor which was expressed only in B Lymphocytes. The OCT-2 factor was identified in neuronal cells [2]. It has been shown that his alternative splicing is regulated differently in different cell types and that it may play an important role in the. (2014) Absence of Expression of OCT-2 in Gastric Cancer Cells. It was demonstrated that OCT-1, another member of the POU homeodomain family of transcription factors that share with OCT-2, the ability to recognize the canonical octamer motif (ATGCAAAT) that regulates the transcription of various genes, is over expressed in intestinal metaplasia and intestinal type carcinomas in human gastric cancer [4]. Some experiments with high-density oligonucleotide microarray analysis suggested that OCT-2 could be overexpressed in human gastric cancer with a significant statistical relationship with lymph node metastasis [5]

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