About the method of creating an infectious background of fusarium for vegetable pea

Abstract

Relevance and methodology . Research was carried out in 2018-2019 in the Moscow region at VNIIO-branch of the Federal state budgetary INSTITUTION FNTSO on the culture of vegetable peas Pisum sativum L. As a result of the assessment of the phytosanitary condition of the collection of pea varieties, it was revealed that the dominant pathogens of both seeds and parts of vegetating plants examined in the field were fungi of the genera Alternaria and Fusarium with a prevalence of 45-65% of colonies isolated in vitro. Using the method of isolation of fungal pathogens from the surface of seeds and seedlings, four isolates of fungi of the genus Fusarium were obtained in pure culture. Five more isolates of Fusarium fungi that cause root rot and withering of plants during the bean formation phase were isolated using the method of isolating a population of pathogens from the organs of vegetating plants. Results . According to cultural and morphological features, four Fusarium isolates were identified as belonging to the species F. oxysporum. Using the method of determining the aggressiveness of isolated isolates on a sandy substrate in vegetation tanks, the most aggressive isolate No. 3 was identified, which will be used in further work on creating an artificial infectious background for the selection of Fusarium-resistant breeding forms of peas. The authors have compiled an up-to-date map of the prevalence and zones of harmfulness in Russia of pea fusariosis, which causes root rot and tracheomycosis wilting.