Abstract
Background This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS. Methods Thirty-eight newly diagnosed MDS patients were enrolled in the Department of Hematology of General Hospital of Tianjin Medical University from June 2015 to June 2016. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. M1 macrophages were cultured in vitro, and the expression of IL-1β and TNF-α mRNA was measured using real-time polymerase chain reaction. Results Compared with the normal control group, the proportion of bone marrow monocytes was higher (2.11 ± 0.93% vs. 3.66 ± 3.38%), and the mean fluorescence intensity of surface molecule CD14 was lower in the higher-risk (HR) MDS group (639.05 ± 359.78 vs. 458.26 ± 306.72, p < 0.05). The ratio of M2 macrophages to monocytes was higher in patients with HR-MDS (1.82 ± 2.47% vs. 3.93 ± 3.81%, p < 0.05). The ratio of M1 to M2 macrophages was lower in the HR-MDS group (3.50 ± 3.22 vs. 1.80 ± 0.88, p < 0.05). The expression of IL-1β and TNF-α mRNA in M1 macrophages was significantly lower in the MDS group (p < 0.05). Conclusions Patients with MDS had abnormal macrophage polarization, which may be involved in the alteration of bone marrow microenvironments.
Highlights
Myelodysplastic syndrome (MDS) is a heterogeneous group of malignant and clonogenic diseases that originate from hematopoietic stem cells
There was no significant difference in the proportion of bone marrow monocytes between the normal control group and the LR group, and the proportion of bone marrow monocytes was significantly higher in the HR group than in the control group (p < 0:05)
There was no significant difference in the median fluorescence intensity (MFI) of CD14+ cells between the normal control group and the LR group, and the MFI of CD14+ cells was significantly lower in the HR group than in the control group (p < 0:05)
Summary
Myelodysplastic syndrome (MDS) is a heterogeneous group of malignant and clonogenic diseases that originate from hematopoietic stem cells. Recent studies have suggested that monocytes and macrophages are part of the bone marrow microenvironment related to homing, mobilization, senescence of hematopoietic stem cells, and the formation of erythropoiesis [4]. This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. Compared with the normal control group, the proportion of bone marrow monocytes was higher (2:11 ± 0:93% vs 3:66 ± 3:38%), and the mean fluorescence intensity of surface molecule CD14 was lower in the higher-risk (HR) MDS group (639:05 ± 359:78 vs 458:26 ± 306:72, p < 0:05). Patients with MDS had abnormal macrophage polarization, which may be involved in the alteration of bone marrow microenvironments
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