Ability to self-assemble of humic acids for production of pH responsive nanoparticles with antioxidant activity and capability for entrapping lipophilic compounds

Abstract

Event Abstract Back to Event Ability to self-assemble of humic acids for production of pH responsive nanoparticles with antioxidant activity and capability for entrapping lipophilic compounds Maria Helena Santana1, Bruna Alice Melo1 and Fernanda Motta1 1 University of Campinas, Department of Materials Engineering and Bioprocesses, Brazil Humic acids (HAs) are macromolecules that comprise humic substances , which are distributed in terrestrial soil, natural water, and sediments, as a result of the decay of vegetable and natural residues . Commercial HAs are extracted from peat and coal. Recently, we demonstrated that HAs could be produced by fungal fermentation using the empty fruit bunch of palm trees as a natural sustainable source . The natural HAs have variable composition, depending on the source and extraction process. Their molecular structure contain numerous groups including carboxylic acid, phenol, enol, alcohol, quinone, ether and others, which grants them multiple functionalities. Natural HAs have been widely used in agriculture as nutrient and fungicidal and also to capture metals in soil and water. HAs also are beneficial to human health mainly as antioxidant, antiinflammatory and for wound healing. Furthermore, the natural muds containing HAS are used as thermal mud-packs in cosmetic treatments and also for alleviate the pain of joins in osteoarthritis. The sustainable and controlled production perspective opens ways for the development of new HAs-based products for pharmaceutical and medical applications. In this context, we explored the ability of HAs for pH-induced deprotonation/deprotonation as a strategy for the controlled production of colloidal nanoparticles, the incorporation of Pluronic 127 surfactant, and also the capabilities these structures as antioxidants and for entrapment of curcimun as a lipophilic compound. The results shown that pH–induced deprotonation/protonation produced nanoparticles with controlled size (100-200nm mean diameter) and zeta potential. Moreover, these structures were able to interact with Pluronic 127, forming flexible nanoparticles able to permeate nanoporous membranes, remaining their integrity. The antioxidant assays have showed that HAs were able to scavenge ABTS ( 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), which was more effective in the presence of PF127. HAs also have shown efficiency higher than 90% for curcumin entrapment, increasing their antioxidant effects in a synergistic manner. These results points out for the feasibility of using HAs or HAs-PF127 nanoparticles in topical treatments as an antioxidant agent and/or as a lipophilic and pH-responsive drug carrier. Keywords: Drug delivery, nanoparticle, stimuli-response, biofunctional molecule Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Nano-structured materials for unique functions Citation: Santana M, Melo B and Motta F (2016). Ability to self-assemble of humic acids for production of pH responsive nanoparticles with antioxidant activity and capability for entrapping lipophilic compounds. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02824 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Maria Helena Santana Bruna Alice Melo Fernanda Motta Google Maria Helena Santana Bruna Alice Melo Fernanda Motta Google Scholar Maria Helena Santana Bruna Alice Melo Fernanda Motta PubMed Maria Helena Santana Bruna Alice Melo Fernanda Motta Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.