Abstract
BackgroundTo investigate on expressions and clinical significances of CD133 protein and vasculogenic mimicry (VM) in primary non-small cell lung cancer (NSCLC).MethodsThe specimens of NSCLC from 305 Chinese patients with follow-up were analyzed for CD133 protein expression and VM by immunohistochemical and histochemical staining.ResultsIn NSCLC, positive rates of 48.9% and 35.7% were obtained for CD133 and VM, respectively. The VM and expression of CD133 were significantly higher in carcinoma than in normal. There were a positive relationship between the VM and expression of CD133 and the tumor grade, lymph node metastasis and clinical stage (all P<0.05). The overall mean survival time of the patients with CD133 and VM positive expression was lower than that of patients with negative expression. Microvessel density (MVD) was positive corresponded with the grade, lymph node metastasis and clinical stage (all P<0.05). The overall mean survival time of the patients with MVD≥22’s group was shorter than that of patients with MVD<22’s group. Pathological-tumor-node-metastasis (pTNM) stage, positive expression of CD133 and VM, postoperative therapy and MVD were independent prognostic factors of NSCLC (P<0.05). Immunohistochemistry revealed an important intratumoral heterogeneity in all four CD133 expression profiles.ConclusionsVM, MVD and expression of CD133 are related to differentiation, lymph node metastasis, clinical stage, and prognosis. It is suggested that CD133, VM and MVD should be considered as a potential marker for the prognosis.
Highlights
To investigate on expressions and clinical significances of CD133 protein and vasculogenic mimicry (VM) in primary non-small cell lung cancer (NSCLC)
The cells external to the lumen of the VM channels were positive for tumor cells, which indicated the channels were formed by NSCLC cells (Figure 5, 6, and 7)
The positive staining of VM and Microvessel density (MVD) score were found to be closely linked to pTNM stages, grade of tumors, lymph node metastasis in NSCLC (P
Summary
To investigate on expressions and clinical significances of CD133 protein and vasculogenic mimicry (VM) in primary non-small cell lung cancer (NSCLC). A major problem in finding treatments is the frequent resistance to drugs which emerges. This is linked to the development and maintenance of small population of tumor cells, termed. Maniotis [29] and his coworkers had described an angiogenesis-independent pathway called “vasculogenic mimicry (VM)”, a new phenomenon in which highly aggressive human melanoma cell mimic endothelial cells and form vascular channel-like structures to convey blood without the participation of endothelial cells. We performed an immunohistochemical investigation to explore the role of the CD133 and VM in chinicopathology and prognosis in 305 cases of NSCLC
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