ABA-INDUCED expression 1 is involved in ABA-inhibited primary root elongation via modulating ROS homeostasis in Arabidopsis

Abstract

To endure environmental stresses, plants have evolved complex regulatory mechanisms involving phytohormones, including abscisic acid (ABA). The function of the plant-specific AT-rich sequence zinc-binding protein (PLATZ) family has not yet been extensively characterized in Arabidopsis (Arabidopsis thaliana). In this report, we evaluated the function of a putative member of the PLATZ family in Arabidopsis, ABA-INDUCED expression 1 (AIN1). We determined that AIN1 expression was induced by ABA and abiotic stresses. AIN1 overexpression (OE) enhanced ABA sensitivity and inhibited primary root elongation, but reduced expression of AIN1 in RNA interference (RNAi) plants produced roots less sensitive to ABA. When treated with ABA, we observed a reduction of meristem size and over-accumulation of reactive oxygen species (ROS) at the root tips of OE lines, demonstrating the importance of AIN1 in plant responses to ABA. A set of ROS scavenger genes showed reduced expression in the OE lines but improved in the RNAi plants relative to Col-0. In addition, we report that exogenous application of reduced glutathione (GSH) rescued the root growth defects seen in AIN1 overexpression lines treated with ABA. In summary, our results suggest that Arabidopsis AIN1 is involved in ABA-mediated inhibition of root elongation by modulating ROS homeostasis.