Abstract
Genome wide analysis of Arabidopsis thaliana reveals a unique genetic arrangement of ACGT motif in the protein phosphatase 2C (PP2C) like promoter (accession number AT5G59220). In the present study, full length, 900 base pair, 500 base pair, 400 base pair and NRM deletion variants of PP2C like promoter were constructed to investigate the activity of PP2C like promoter in the presence of inducers like abscisic acid, jasmonic acid and salicylic acid. The PP2C promoter has three ACGT elements in close vicinity. They are so positioned that they are separated by a spacer of 30 base pair and 5 base pair respectively from each other. The study has shown that ACGTN30ACGT genetic architecture is essential for the promoter to be induced in response to abscisic acid. The synergistic and antagonistic effects of cis elements were observed. AACA is a positive regulatory element in endosperm and is known to act as negative regulatory element in other tissues. In this study AACA, have been found to negatively regulate the expression of reporter gene EGFP in both induced and under uninduced conditions.
Highlights
Plants, being sessile in nature need to adopt different methods to combat stresses in comparison to animals, be it environmental or biological
For analyzing the presence of cis elements in full length of phosphatase 2C (PP2C) like promoter, the PLACE database was used [21,22]. This analysis shows that different motifs responsive to inducers like abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), light regulation etc. are present in the PP2C like promoter (Table 2)
Up regulation and down regulation of expression is based on the synergistic action of cis elements present in the promoter region of the gene
Summary
Plants, being sessile in nature need to adopt different methods to combat stresses in comparison to animals, be it environmental or biological. The ABA dependent stress responsive genes have ABA responsive element (ABRE) which is present in its promoter region [2] and has ACGT as a core sequence [3]. ACGT is the core sequence of the G box, involved in the red light signalling [4], UV response, anaerobiosis in plants and SA response elements [5]. The ABA mediated stress signaling pathway involves the receptor PYL/PYR/RCAR, a negative regulator PP2C, positive regulator SnRK2s, cis regulatory element known as ABRE and transcription factors [6]. Cis element is a consensus sequence present in the upstream region of the basal promoter, and it acts as the binding site for transcription factors. Gene expression is the result of the interaction between cis element and its complementary transcription factor, the latter being activated by a particular developmental stage or environmental cues
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
