PP2C-like Promoter and Its Deletion Variants Are Induced by ABA but Not by MeJA and SA in Arabidopsis thaliana.

Purva Bhalothia,Rajesh Mehrotra,Anshu Alok,Chetna Sangwan,Sandhya Mehrotra

doi:10.3389/fpls.2016.00547

Abstract

Gene expression is mediated through interaction between cis regulatory elements and its cognate transcription factors. Cis regulatory elements are defined as non-coding DNA sequences that provide the binding sites for transcription factors and are clustered in the upstream region of genes. ACGT cis regulatory element is one of the important cis regulatory elements found to be involved in diverse biological processes like auxin response, salicylic acid (SA) response, UV light response, ABA response and jasmonic acid (JA) response. We identified through in silico analysis that the upstream region of protein phosphatase 2C (PP2C) gene has a distinct genetic architecture of ACGT elements. In the present study, the activation of the full length promoter and its deletion constructs like 900 base pair, 500 base pair, 400 base pair and NRM (Nathji Rajesh Mehrotra) were examined by stable transformation in Arabidopsis thaliana using β-glucuronidase as the reporter gene. Evaluation of deletion constructs of PP2C-like promoter was carried out in the presence of phytohormones like abscisic acid (ABA), SA and JA. Our result indicated that the full length and 900 base pair promoter-reporter constructs of PP2C-like promoter was induced in response to ABA but not to methyl jasmonate and SA.

Highlights

Abiotic and biotic stresses drastically affect the plant growth and productivity
PP2Clike promoter was characterized by making a series of 5 deletion constructs like 900 bp, 500 bp, 400 bp and NRM, followed by analysis of cis regulatory using PLACE and PlantCARE databases (Higo et al, 1999; Lescot et al, 2002)
Further cis elements exist as dyads within a specific distance from one another, for example, ABA responsive element (ABRE)– ABRE or ABRE-coupling element (CE) required for abscisic acid (ABA) response (Gomez-Porras et al, 2007)

Summary

Introduction

Abiotic and biotic stresses drastically affect the plant growth and productivity. In response to these challenges plants adapt themselves through various mechanisms which are controlled by the molecular, physiological and cellular processes (Mehrotra et al, 2014). Phyto hormones such as ABA, SA, JA, and ethylene (ET) tend to play a pivotal role in helping plants to adapt themselves to abiotic and biotic adversities (Fujita et al, 2006). The CaMV-35S, a constitutive promoter is extensively used in plant genetic engineering. Over expression of stress responsive genes under the control of such

Methods

Results

Conclusion