Abstract
Chloramphenicol (CAP) is a kind of broad-spectrum antibiotic, which has been forbidden in food by most countries because of its side effects. In this study, a simple and low-cost biosensor for CAP detection in food was developed. The biosensor consisted of an aptamer specific to CAP and a pair of split probes that could self-assemble as DNAzyme. The detection result could be identified by the naked eye and the visual limit was 10nM CAP. The absorbance of final reaction products at 417nm had a linear relationship with the logarithm of the CAP concentration in a range from 10 to 200nM, and the limit of detection was 87.3pM. The visual analysis by imageJ also showed a linear detection range between 25 and 200nM. The entire detection procedure could be completed in about 1.5h at a cost of about 0.16 dollars per reaction. We believe that the biosensor shows great potential in the rapid and sensitive detection of CAP in food.
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