Abstract
We used surface-enhanced Raman scattering (SERS) for the quantitative and sensitive detection of chloramphenicol (CAP). Using 30 nm colloidal Au nanoparticles (NPs), a low detection limit for CAP of 10−8 M was obtained. The characteristic Raman peak of CAP centered at 1344 cm−1 was used for the rapid quantitative detection of CAP in three different types of CAP eye drops, and the accuracy of the measurement result was verified by high-performance liquid chromatography (HPLC). The experimental results reveal that the SERS technique based on colloidal Au NPs is accurate and sensitive, and can be used for the rapid detection of various antibiotics.
Highlights
Chloramphenicol (CAP), a common broad-spectrum antibiotic, is widely used in animal husbandry and aquaculture for its efficient inhibition of bacterial growth
The 10 nm colloidal Au NPs were prepared in the following method: 1.5 mL 1% w/v sodium citrate and 42.5 μL 0.1% w/v AgNO3 were added into 1 mL 20 mM HAuCl4 aqueous solution successively under vigorous stirring, and after stirring for 5 min the mixed solution was added into 47.5 mL boiling deionized water
transmission electron microscopy (TEM) was used to characterize the structures of the colloidal Au NPs
Summary
Chloramphenicol (CAP), a common broad-spectrum antibiotic, is widely used in animal husbandry and aquaculture for its efficient inhibition of bacterial growth. Even after being discharged into the environment, it cannot degrade in the short-term [3,4], resulting in a lasting environmental pollution. For these reasons, CAP has been banned from animal feed and treatment in many countries, including China, the USA, Australia, and many European countries [5,6]. Various SERS substrates have been designed by researchers to meet the need for substance detection, and they display a variety of testing performances; these include silver nanoparticles (NPs) [17], graphene/silver/laser-textured Si [18], and Au core-Ag shell NPs film [19]. Colloidal Au NPs were used as SERS-active substrate to detect the CAP concentration in several types of CAP eye drops, and the accuracy of the testing results was verified by HPLC
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