Abstract
A novel twin-trough developing tank is described. The bottom of the tank is separated into two troughs so that the amount of solvent can be reduced to approximately 20 ml, which is sufficient to saturate the tank and develop the chromatogram. After development, practically no solvent remains in the trough. A revision or change of existing thin-layer chromatographic methods is usually not necessary; the results remain unchanged. Compared with the conventional tank, employing 100 ml of solvent, the new tank has the following advantages: a saving of 50 to 80% on solvents; the avoidance of solvent waste ( i.e., a contribution by analysts towards environmental protection); “automatic” adjustment of the solvent development distance owing to the amount of solvent chosen; and an improvement in the separation and its reproducibility by means of layer pre-loading or by adjustment of the relative humidity in the tank.
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