Abstract
A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.
Highlights
Hemorrhoids, known as piles, are swollen veins located around the anus or in the lower rectum
Literature review revealed that UV, HPLC, high-performance thin layer chromatography (HPTLC) methods have been reported for the estimation of individual phytoconstituents, BER and GZ in crude and herbal formulation & LC-MS, LC-MS-MS have been reported for the estimation in biological fluid [2, 3]
Any method has not been reported for the simultaneous estimation of both the phytoconstituents from herbal formulation
Summary
Hemorrhoids, known as piles, are swollen veins located around the anus or in the lower rectum. It can either be internal or external. Commiphora wightii (Guggulu) is known for its active ingredients guggulsterones (GE& GZ) It has astringent, antiseptic with anti-inflammatory and demulcent properties that helps to reduce the pain and inflammation. It is found to have antibacterial, anti-inflammatory and laxative properties and is useful in hemorrhoids [4]. Any method has not been reported for the simultaneous estimation of both the phytoconstituents from herbal formulation.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Pharmaceutical Technology, Research and Management
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
