Abstract
A Brassica juncea line carrying an introgression from Moricandia arvensis restored male fertility to two cytoplasmic male-sterile (CMS) B. juncea lines carrying either M. arvensis or Diplotaxis catholica cytoplasm. Genetics of fertility restoration was studied in the F1, F2, F3 and backcross generations of the cross between CMS and fertility-restorer lines. No male-sterile plants were found in F1-F3 generations of the cross between CMS [M. arvensis] B. juncea and the restorer. However, a 1: 1 segregation for male sterility and fertility was observed when the F1 was pollinated with non-restorer pollen from a euplasmic line. These results clearly show that restoration is mono-genic and gametophytic. In CMS lines carrying D. catholica cytoplasm, the restorer conferred male fertility to the F1 and showed 3: 1 and 1: 1 segregations for male fertility and sterility in F2 and BC1 generations, respectively, indicating a monogenic, sporophytic mode of fertility restoration. The results were also supported by pollen stainability in the F1 which was about 65% in M. arvensis-based CMS and >90% in D. catholica-based CMS. The above results are discussed in the light of previous molecular studies which showed association between CMS and atpA in both systems.
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