A Two-dimensional Electrophoretic Analysis of Protein Synthesis and Accumulation during Adventitious Shoot Formation in Somatic Tissue Cultures of Solanum carolinense L.

Abstract

Adventitious shoot formation from tissue cultures of Solanum carolinense can be induced when stem segments are cultured on medium containing benzyladenine. When cultured on medium containing 2,4dichlorophenoxyacetic acid, stem explants produced only callus. Using two-dimensional gel electrophoresis, total soluble protein and protein labeled in vivo with [ 35 S]-methionine were extracted from regenerating and nonregenerating cultures and the gels or autoradiographs computer analyzed to identify different sets of proteins associated with development in vitro . Overall, the level of expression of 51 stained and 40 labeled proteins was altered, and it was shown that in vitro morphogenesis of stem segments of S. carolinense is associated with major quantitative changes in protein expression. Qualitatively, stagespecificpolypeptides related to callus formation and in vitro organogenesis were also identified.