A twin rhizospheric bacterial consortium induces systemic resistance to a phytopathogen Macrophomina phaseolina in mung bean

Abstract

The two bacterial isolates, Pseudomonas putida CRN-09 and Bacillus subtilis CRN-16, were isolated, characterized and identified as potential plant growth promoting rhizobacteria (PGPR) of Vigna radiata on the basis of different plant growth promoting (PGP) portrayals. The synergistic interaction between both the isolates was evaluated to develop biocoenotic consortium. The consortium showed a significant enhancement in seed germination by 18.73% in mung bean plants. Both the isolates and their consortium induced systemic resistance (ISR) in V. radiata under disease-prevalent conditions. The expression of ISR by a twin bacterial consortium against Macrophomina phaseolina was conferred substantially due to enhanced levels of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), β−1,3 glucanase and chitinase. The results postulate the putative role of the consortium of Pseudomonas putida CRN-09 and Bacillus subtilis CRN-16 in exploiting the development of plant immunity.