Abstract
An improved technique for purifying Plasmodiophora brassicae resting spores from host root tissue and other contaminants is described. After extraction from macerated infected Chinese cabbage roots by a series of centrifugations in 50% sucrose and distilled water, P. brassicae resting spores were separated from other contaminants in a continuous gradient of LUDOX (40% silica suspended in NaOH, pH 9.8; DuPont)/deionized water. This purification technique produces clean suspensions of P. brassicae resting spores suitable for propagation of the organism, long-term storage, and extraction of DNA or other molecules of interest.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.