Abstract
Nuclear receptor subfamily 5 group A member 2 (NR5A2), also referred to as LRH-1 or FTF, is an orphan nuclear hormone receptor that is involved in regulating embryonic development, ovarian granulosa cell differentiation, gonadal sex differentiation, and steroidogenesis in mammals. However, little is known about how NR5A2 regulates reproduction in sheep. In this study, we amplified the promoter sequence of NR5A2 and determined that its core promoter region ranged from -721 nt to -281 nt. A T > G polymorphism at -700 nt was detected in the core promoter region. Association analysis found that the litter sizes of Hu ewes at their second and average parities with genotype GG (2.20 ± 0.20 and 1.97 ± 0.06, respectively) were significantly higher than those of ewes with genotype TG (1.68 ± 0.10 and 1.74 ± 0.05, respectively) (p < 0.05) and TT (1.67 ± 0.10 and 1.62 ± 0.06, respectively) (p < 0.05). The litter size of Hu ewes at their third parity with genotype GG (2.10 ± 0.10) was significantly higher than that of ewes with genotype TT (1.56 ± 0.12) (p < 0.05). A luciferase assay showed that the -700G allele increased the luciferase activity relative to the -700T allele. Furthermore, the -700T > G polymorphism created a novel binding site for metal-regulatory transcription factor 1 (MTF-1). A competitive electrophoretic mobility shift assay confirmed that MTF-1 specifically bound with the G-type promoter of NR5A2. An overexpression experiment demonstrated that MTF-1 was involved in the alteration of NR5A2 transcription activity and further increased NR5A2 gene mRNA expression. Our findings revealed that the -700T > G polymorphism promoted NR5A2 expression due to the positive effects on NR5A2 gene transcription activity by MTF-1 and thereby increased fecundity in Hu sheep.
Highlights
Nuclear receptor subfamily 5 group A member 2 (NR5A2), referred to as liver receptor homolog-1 (LRH-1) or fetoprotein transcription factor (FTF) in Drosophila (Galarneau et al, 1996), is an orphan nuclear hormone receptor
The difference in luciferase activity was not statistically significant between pGL3-721 and pGL3-1373 (p > 0.05), which revealed that the location of the core promoter region of the ovine NR5A2 gene ranged from ‐721 to ‐281 bp (Figure 1)
These results indicate that the ‐700T/G polymorphism in the core promoter region of NR5A2 gene was involved in regulating the litter size of Hu sheep
Summary
Nuclear receptor subfamily 5 group A member 2 (NR5A2), referred to as liver receptor homolog-1 (LRH-1) or fetoprotein transcription factor (FTF) in Drosophila (Galarneau et al, 1996), is an orphan nuclear hormone receptor It is widely expressed in all tissues, especially in the ovaries (Falender et al, 2003; Li et al, 2015), and is involved in embryonic development (Wei et al, 2018), Promoter SNP Upregulates NR5A2 Activity ovarian granulosa cell differentiation (Meinsohn et al, 2017), gonadal sex differentiation (Yan et al, 2018), and steroidogenesis (Camats et al, 2015; Xiao et al, 2018). This suggests that the NR5A2 gene plays an important role in regulating the reproductive performance of female animals
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