A Systematic Study on Mirror Repeats in CTLA4 and KCNJ11 Genes of Diabetes

Abstract

All cellular functions are controlled by the regulatory material which is constituted majorly by variety type of repeat elements. Among that types a unique pattern of bases form a Mirror repeat (MR) which thought to plays variety of roles at the genomic level that include regulation of gene expression, genomic integrity, triplex structure formation etc. The present research focused on identification of Mirror repeats in different genes types involved in both diabetes types. Two different genes CTLA4 (T1D) & KCNJ11 (T2D) were targeted to identify these sequence type. A manual method refers as FPCB is utilized to extract out MR sequences. The targeted gene sequences were divided into short regions so that it can be handled out easily while bioinformatics based analysis. Among the both types it was reported that both genes have different pattern of distribution of MR sequences. CTLA4 have 346 MR sequences in total in which MR235 have highest length of 50bps. In case of KCNJ11 a total no of 235 MR sequences were reported in which MR105 having highest length of 58bps. The present study will be helpful to elucidate the roles of MR sequences in diseases development & progression. It will be helpful for sequence based protein finding studies using bioinformatics tools as well as wet lab experiments.