Abstract

Morels (Morchella spp.) are one of the most highly prized edible mushrooms found in the wild of North America. Because environmental conditions can cause members of the genus to vary morphologically, the number of species has been disputed. Some au? thors classify the genus into three to five species, while others argue for as many as 50 species. DNA from lines of Morchella and a closely related genus (Verpa) was isolated. The large subunit of the ribosomal DNA repeat was amplified using the polymerase chain re? action and then digested with restriction enzymes. Re? striction fragment length polymorphisms were found among the lines investigated and used to separate the lines into genotypic classes. More genetic variation may occur between geographically isolated popula? tions of the same species than between two putatively distinct species. The phylogenetic tree developed from restriction data suggests that the black morels (M. an- gusticeps, M. elata, and M. conica) and the yellow morels (M. esculenta, M. crassipes, and M. deliciosa) are separate taxonomic groups.

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