A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

G Baillie,M.D Owens,G.W Halbert

doi:10.1016/s0022-2275(20)30188-7

Abstract

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.

Highlights

A synthetic LDL has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain
A microemulsion with a similar lipid composition to LDL but with no amphipathic peptide containing the apoprotein B (apoB) receptor domain was similar to Delipidated fetal calf serum (DFCS)
The PEP1sLDL induced increase was significantly greater (P Ͻ 0.05) than that produced by control microemulsion, an identical system but with no amphipathic peptide

Summary

Introduction

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. SLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application. A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro.

Methods

Results

Conclusion