A synthetic community of siderophore-producing bacteria increases soil selenium bioavailability and plant uptake through regulation of the soil microbiome

Abstract

Dietary selenium (Se) is an effective strategy to meet Se requirement of human body, and Se biofortification in crops in seleniferous soils with selenobacteria represents an eco-friendly biotechnique. In this study, we tested the effectiveness of siderophore-producing bacterial (SPB) synthetic communities (SynComs) in promoting plant Se uptake in a subtropical seleniferous soil where the fixation of Se by ferric-oxides is severe. The results indicated that SPB SynComs drastically elevated soil bioavailable Se content by up to 68.7 %, and significantly increased plant Se concentration and uptake by up to 83.1 % and 92.2 %, respectively. Seven out of ten SPB isolates in the SynComs were enriched in soils after 120 days of inoculation. Additionally, variation partitioning analysis (VPA) revealed that the contribution of soil bacterial community (up to 42.8 %) to the increased plant Se uptake was much greater than that of soil bioavailable Se (up to 5.1 %), suggesting a direct pathway other than the pathway of mobilizing Se. The relative abundances of some operational taxonomic units (OTUs) showed significantly positive relationship with plant Se status but not with soil Se status, which supports the results of VPA. Network analysis indicates that some inoculated SPB isolates promoted plant Se uptake by regulating the native bacterial taxa. Taken together, this study demonstrates that SPB can be used in Se biofortification in crops, especially in subtropical soils.