Abstract
The objective of this paper was to study the in vitro anti-breast cancer activity of polysaccharides from Radix ranunculus ternati. Different concentrations of polysaccharide extracts were selected, and MTT assay and flow cytometry (FCM) were used to investigate their growth-inhibitory and apoptosis-inducing effects on human breast cancer MCF-7 cell lines. Radix ranunculus ternati polysaccharides had varying degrees of effects on the growth of human breast cancer MCF-7 cell lines, and the differences were significant compared with the blank control group. FCM showed that the polysaccharides can induce apoptosis. In addition, it can also enhance NK cell activity. Radix ranunculus ternati polysaccharides have a relatively good in-vitro anti-breast cancer activity.
Highlights
Radix ranunculus ternati is the dried root tuber of Ranunculus ternatus Thunb. in the family Ranunculaceae (Pharmacopoeia of the People's Republic of China, 2000), which is known as Sansancao (Zhejiang), Maozhuaercao (Henan), Maozhuazi (Henan), Yajiaoban (Anhui) and Jinhuacao (Guangxi) (Xie et al, 1996; Yu et al, 1996)
Each Radix ranunculus ternati polysaccharides dose group could inhibit the growth of human breast cancer MCF-7 cell lines in varying degrees
One study has considered from the perspective of trace elements and health that Cu/Zn ratio increases in serum of cancer patients, while the Cu/Zn ratio trend of Radix ranunculus ternati polysaccharides is opposite to that of cancer patients, and may help regulate the Cu/Zn balance of patients, thereby exerting an anti-cancer effect (Quan et al, 1997; Wang et al, 1997; Zheng et al, 1997)
Summary
Radix ranunculus ternati is the dried root tuber of Ranunculus ternatus Thunb. in the family Ranunculaceae (Pharmacopoeia of the People's Republic of China, 2000), which is known as Sansancao (Zhejiang), Maozhuaercao (Henan), Maozhuazi (Henan), Yajiaoban (Anhui) and Jinhuacao (Guangxi) (Xie et al, 1996; Yu et al, 1996). In the family Ranunculaceae (Pharmacopoeia of the People's Republic of China, 2000), which is known as Sansancao (Zhejiang), Maozhuaercao (Henan), Maozhuazi (Henan), Yajiaoban (Anhui) and Jinhuacao (Guangxi) (Xie et al, 1996; Yu et al, 1996) It is mainly grown in Xinyang, Hengchuan and Xixian of Xinyang Region as well as Zhengyang and Queshan of Zhumadian Region in Henan. Radix ranunculus ternati is often decocted with water for medicinal use, and the initial pre-test revealed a relatively high content of polysaccharides in Radix ranunculus ternati In this experiment, polysaccharides were extracted from Radix ranunculus ternati, and its effect on in vitro-cultured human breast cancer MCF-7 cell line was studied, so as to find effective antitumor parts of Radix ranunculus ternati and their mechanisms of action, and provide a theoretical basis for its clinical treatment of cancer
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