Abstract
This paper studies the effects of Lobelia chinensis on colon precancerous lesions and on colonic epithelial proliferation and apoptosis in DMH-induced rats. After two weeks of feeding, 50 Wistar rats were randomly divided into five groups, namely the normal group, model group, Lobelia chinensis low-dose group, medium-dose group and high-dose group. Lobelia chinensis was made into ACF model, and administered to experimental groups for 10 consecutive weeks. Control group was given equivalent amount of normal saline. After feeding for 10 weeks, the rats in each group were sacrificed and the changes in colonic ACF number of rats in experimental groups were observed, and the inhibition rates were calculated. The results showed that among the rats fed for 24 h and 48 h, the number of apoptotic cells in colonic crypts of rats in DMH group did not differ significantly from the control group, while the difference was obvious between the control group and Lobelia chinensis treatment groups. The medium and high doses, that is, 0.45 g/kg and 1.35 g/kg can significantly inhibit ACF formation (P<0.01). The inhibition rates of low, medium and high doses were 8.12%, 59.42% and 65.44%, respectively.
Highlights
Colorectal cancer is a malignancy with evident genetic predisposition. 25% of patients with colorectal cancer have a family history; 3%-5% of the cancer patients present obvious “hereditary colon cancer syndromes”, including the familial adenomatous polyposis (FAP) and hereditary non-polyposis colorectal cancer (HNPCC) (Zhao, 2010)
The difference was obvious between the control group and Lobelia chinensis treatment group of which the number of apoptotic cells corresponding to the medium and high doses was statistically significantly different compared with the control group, while the low dose only had a slight change, which was not significantly different
Among the aqueous solutions of three different concentrations prepared by the Lobelia chinensis aqueous extract powder, the inhibition rate of low-dose group was significantly lower than medium- and high-dose groups
Summary
Colorectal cancer is a malignancy with evident genetic predisposition. 25% of patients with colorectal cancer have a family history; 3%-5% of the cancer patients present obvious “hereditary colon cancer syndromes”, including the familial adenomatous polyposis (FAP) and hereditary non-polyposis colorectal cancer (HNPCC) (Zhao, 2010). It is reported in the literature that the compound Shenqitang can inhibit the metastasis and progression of human colon cancer cells in nude mice, and Lobelia chinensis is one of its ingredients (compound Shenqitang: Radix Angelicae Sinensis, Radix Aucklandiae, Pericarpium Citri Reticulate, Radix Glycyrrhizae, Poria cocos, ginseng, Radix Astragali, Radix Notoginseng, Lobelia chinensis, Sculellaria barbata, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata, Kusnezoffii fried Guya, Fructus Oryzae Germinatus, Fructus Hordei Germinatus) ( Shen et al, 2003) This experiment selected the DMF-induced Wistar rat colon carcinogenesis model to observe the effect of aqueous extract of Lobelia chinensis on colon precancerous lesions in rats after administration.
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