Abstract
Abstract This study was to discuss the relationship among the change in the activity of Bombyx mori carboxylesterase (BmCarE) in the midguts, the differential expression of BmCarE gene ( bmcare) in the midguts, and the ability of Bombyx mori resistant to densonucleosis virus (BmDNV), and to elucidate the molecular mechanism of resistance to BmDNV-Z. With two silkworm strains, HUABA, which is susceptible to BmDNV-Z, and BC 8 (a near isogenic line of HUABA), which is completely resistant to the same virus, as materials, the activity of BmCarE in the midgut was determined by Bio-Tek Synergy, and the differential expression of bmcare between the two strains was investigated by real-time fluorescence quantitative PCR, both at 12, 36, and 72 h post oral inoculation of the two strains with virus (hereafter referred as inoculation). While the activity of BmCarE in the midguts of BC 8 inoculation group at 12 h post inoculation was higher than that in the BC 8 control group, the HUABA inoculated group, and the HUABA control group by 3.28, 2.26. and 3.02 times, respectively, with the difference being highly significant ( P < 0.01), there was no statistical difference among the other groups. The relative expression level of bmcare in the midguts of BC 8 inoculation group at 12 h post inoculation was higher than that in the BC 8 control group, the HUABA inoculation group, and the HUABA control group by 17.714, 21.76. and 15.09 times, respectively, with the difference being highly significant ( P < 0.01), and there was no statistical difference among other groups. The elevation of BmCarE activity and expression level of bmcare in the resistant strain at 12 h post inoculation may relate to the resistant gene ( nsd/nsd) and the stimulation of BmDNV-Z. The molecular basis for the elevation of BrnCarE activity in the resistant strain BC 8 may be the change in the expression level of bmcare.
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