A study on molecular mimicry-based glioma vaccine in C6/SD rat model

Abstract

Objective To explore the therapeutic effect and mechanism of molecular mimicry-based vaccine in C6/SD rat model. Methods Mixed vaccines were prepared containing allogeneic 9L cells and cell lysates and syngeneic C6 cell lysates. C6 glioma cells were implanted into SD rats to establish the animal model. A total of 39 rats were randomly divided into 100, 000 treatment group, 200, 000 treatment group and control group. Subcutaneous vaccination were performed at 8 d post modeling and the survival time of rats was observed. MRI examination was taken at 7, 14, 21, 28, 35, 70 d and the tumor volumes were measured. At 20 d, 3 rats in each group were randomly selected for HE staining and immunohistochemical staining, and expressions of Fascin, HS1, MMP-2, Podoplanin, CD8 and CD4 were assessed. Results The survival time in the 100, 000 and 200, 000 treatment groups (29.0 d and 47.5 d, respectively) was longer than that in the control group (18.5 d) (P 0.05). The tumor volume in treatment group first increased and then gradually decreased. At 14 d and 21 d, the mean volumes of tumor in 100, 000 and 200, 000 treatment groups [14 d: (220.68±45.92) mm3, (164.98±45.36) mm3, respectively; 21 d: (426.48±39.69) mm3, (376.23±43.89) mm3, respectively] were less than that in the control group [14 d: (265.30±40.57) mm3; 21 d: (515.64±15.28) mm3] (all P 0.05). At 20 d, the glioma cell density in the treatment groups was lower than that in the control group. The expression levels of Fascin, HS1, MMP-2 and Podoplanin in the treatment group were lower than that in the control group, while the expressions of CD4 and CD8 in treatment groups were higher than those in the control group. Conclusion The molecular mimicry-based vaccine could induce immune response to intracranial glioma in tumor bearing rats, thereby reducing tumor volume and invasiveness and prolonging the survival time of rats. Key words: Glioma; Molecular mimicry; Vaccines; Rats