A study on in vitro culture of Trichuris ovis in different physiological solutions at constant temperature, 37°C

Abstract

The primary aim of invitro culture of whipworms (Trichuris ovis) is to provide artificial conditions under which the life cycle of the parasites completed outside the host under controlled conditions. The physiological solutions used for the present study were sodium chloride (0.64%), Ringer's solution, Tyrode's solution, and Lock-Lewis solution. Parasites were collected from freshly slaughtered intestine of the host. The recovered parasites were washed with running tap water after that with normal saline. After washing parasites were put in four petridishes containing different physiological solutions. Observations were recorded after interval of every 8h. The hundred percent survival of Trichuris ovis was observed at 32, 40, and 48h in NaCl (0.64%), Ringer's, Tyrode's, and Lock-Lewis solution, respectively in case of both male and female parasites. In sodium chloride solution (0.64%) cent percent mortality was observed after 64h of incubation in males and in case of females it was observed 72h. In Ringer's solution cent percent mortality was observed after 72 in males and in females it was observed 80h. In Tyrode's solution cent percent mortality was observed after 88h in males and 96h in females. In Lock-Lewis solution cent percent mortality was observed after 96h in case of both the male and female parasites. Present study could be used to understand the effects of various drugs on the above parasites and also other intra-intestinal parasites.