A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System

Sabrina Resky Pratiwi,Dini Rusdayanti Putri,Nur Ainiah,Hardyanti Hardyanti,Emilia Utomo

doi:10.20956/ijab.v1i1.2269

Sabrina Resky Pratiwi, Dini Rusdayanti Putri + Show 3 more

Open Access

https://doi.org/10.20956/ijab.v1i1.2269

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Abstract

IntroductionEarthworms (Lumbricus rubellus) extract is known to contain bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome. MethodsEarthworms powder was macerated using 50% ethanol for 3 days. The extract was formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible.ResultsThe results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol.ConclusionsThe highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%.Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.

Highlights

International Journal of Applied Biology is licensed under a International Journal of ApplCieredatBivioe loCgoym
By3 previous studies which showed that earthworms has antibacterial
In3 order to[3] find out the highest entrapment efficiency of[3] earthworms extract

Summary

Introduction

International Journal of Applied Biology is licensed under a International Journal of ApplCieredatBivioe loCgoym, mpo-InSsSANtt:rib2u5ti8o0n-244.010Inet-eIrSnaStNion:al25L8ic0en-2se[1,19]. & Abstract& & Earthworms[3] (Lumbricus) rubellus)[3] extract[3] is3 known[3] to3 contain[3] bioactive3 & & protein[3] as3 antibacterial[3] compounds.One[3] of3 the[3] disadvantages[3] of3 polar[3] Provide[3] the[3] highest[3] entrapment[3] efficiency[3] of3 the[3] ethosome.[3] Earthworms3 & efficiency[3] was[3] conducted[3] by3 measuring[3] the[3] amount[3] of3 active[3] protein[3] of3 & earthworms[3] extract[3] that[3] was[3] entrapped[3] in3 ethosome[3] vesicles[3] using[3]

Results

Conclusion