Abstract
A Study of Xylene Free Hematoxylin and Eosin Staining Procedure
Highlights
The diagnostic armamentarium of a pathologist includes histopathological diagnosis of various lesions by the examination of biopsy tissues submitted
The most commonly used method of processing is the formalin fixed, paraffin embedded method with the sections being stained by Haematoxylin and Eosin (H&E) stains
Ankle MR and Joshi PS in their study to evaluate efficacy of xylene free hematoxylin and eosin staining procedure utilized diluted dish washer solution to eliminate xylene and alcohol in their staining protocol. They concluded that xylene and methanol free H and E staining was a better alternative compared to the conventional method. In their study they noted that as many as 90% of sections were stained adequately for offering a diagnosis in the xylene free method compared to 88.33% in the conventional method
Summary
The diagnostic armamentarium of a pathologist includes histopathological diagnosis of various lesions by the examination of biopsy tissues submitted. The aforementioned schedule of tissue processing, sectioning and staining entails the use of various chemicals like formalin, xylene and alcohol. Many of these chemicals are known to be irritants, toxic and carcinogenic. [2,3,4] Xylene and alcohol are commonly used in the H&E staining procedure for the process of dewaxing, clearing and dehydration.[1] The exposure of the histotechnician to the toxic chemicals like xylene and alcohol is maximum during the process of H&E staining and efforts are on to reduce this occupational hazard.[1,2,3,4]. Methods: One hundred paraffin embedded sections each were stained by both conventional method and xylene free method using liquid dish washer. More studies using modifications of the above technique can be undertaken to improve the quality of stained sections eliminating occupational hazards
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