Abstract
Some parameters related to the bidimensional interactions between ceramide (CER) and diolcoylphosphatidylcholine (DOPC) molecules, alone and in mixtures of different weight fractions, were deduced from the study of spreading monolayers at the air-water interface. Spreading isotherms were recorded using both 0.1 M NaCl (pH 5.6) and a phosphate buffer solution (pH 7) as subphases in the 15–30°C temperature range. The bidimensional phases of the two components were obtained and their bidimensional miscibility was deduced as a consequence of positive deviations of the surface areas from the additivity relationship and by applying the phase rule at the collapse pressure. Excess surface Gibbs energies, enthalpies and entropies of mixing were also computed. The results obtained showed that the two components were miscible under the experimental conditions at all temperatures investigated, using both the Na +-ion-containing subphase and the buffered one.
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