Abstract
The acid and alkaline phosphatase activities at different organic loadings in an anaerobic plug-flow digester and a UASB digester were determined together with volatile acid concentrations, pH values, etc. Increases in alkaline and acid phosphatase activity (especially in acid phosphatase) were found in both digesters to be overfed to the level of souring and failure. These increases were observed up to 10 days before the occurrence of more volatile fatty acids production and lower pH values. Phosphatase activity in the digesters appears to be a rapid biochemical test for crediting the instability or failure of anaerobic digestion.
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