Abstract
Mammalian cells show a marked simplification of glycolipids when transformed spontaneously or by oncogenic DNA or RNA viruses. This chapter discusses a study of glycosphingolipids in cultured cell lines from human colonic tumors and fetal intestines. In the study, glycosphingolipids from three cultured human colonic cell lines, (SKCO-1, HT-29, and SW 480) and two lines of human fetal intestine were studied. Glycolipids were radioactively labeled. They were washed with PBS, reduced with NaB 3 H 4 for 10 min, and then washed extensively and harvested. The upper phase was subjected to DEAE-Sephadex column chromatography to obtain ganglioside and neutral glycolipid fractions having five or more sugar residues. The lower phase was subjected to Florisil column chromatography, followed by DEAE-Sephadex column chromatography, to obtain neutral glycolipid and sulfated glycolipid fractions.
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