A study of cobalt (II) complexes involved in marine biogeochemical processes: Co(II)-1,10-Phenanthroline and Co(II)-1,10-Phenanthroline-L-α-Phosphatidylcholine

Abstract

The cell membrane is structured so that the surface layer is composed of lipid molecules with selective permeability for micronutrients and organic ligands. Binding of Co (II) to natural lipid phosphatidylcholine (PC) has been studied to identify a possible mechanism of Co (II) entry through the cell membrane of the biota in detail, by voltammetry followed by checking the system at the air–water boundary, by Langmuir method. Binding of cobalt (II) ions to the PC molecules was enabled by the Co(II)-1,10-Phenanthroline (Phen) complex formation as an intermediate. Co(II)-Phen-PC complex reduction was recorded in the pH range from 5 to 9.5. The reduction was identified as a two-electron irreversible reaction at about −1.5 V, with the reactant adsorption followed dissociation (EC mechanism). The Co(II)-Phen-PC complex electrode surface concentration (Γ) was calculated to be (1.45 ± 0.12) × 10-10 mol.cm−2. Conditional stability constants log KCo(II)Phen2PC = 23.02 ± 0.26 and log KCo(II)Phen2PC2 = 29.31 ± 0.17 (Ic = 0.55) were calculated by CLE/ACSV method. Pressure-area (π-A) isotherms obtained at water–air interface by Langmuir monolayer technique indicated penetration of Co(II)-Phen into the PC monolayer, supporting electrochemical results. The equilibrium constants of the Co (II)-PC system (1:1) at the air–water interface was calculated to be K1 = 2.4 × 10-2 m3 mol−1, while for Co(II)-Phen-PC K2 = 4.86 × 1010 m2 mol−1.