Abstract
It is difficult to stain Hevea latex vessels and other bark parenchyma cells differentially. A method to identify the latex vessels by staining the coagulated latex in them is described here. Hevea bark, on removal, was immediately fixed in formal-acetic-alcohol for 24–36 h, then dehydrated in tertiary butyl alcohol and embedded in paraffin wax, mp 58d`C. A few drops of oleic acid were added to the wax to retain the coagulated latex. The wax ribbons were mounted on slides and dewaxed in Coplin jars containing a 55:45 mixture of toluene and absolute ethyl alcohol at 60d`C for 11/2 h. The sections were then rinsed in 95% ethyl alcohol and stained with the following solutions, all made up in absolute ethyl alcohol: (a) Old bark: 2 ml 2% oil red 4B, 3 ml 1% methyl green, 3 ml 2% light green, and 2 ml saturated aniline sulfate; (b) Young bark: 6 ml 2% oil red 4B, 4 ml 2% light green, 2 ml 10% safranin, 1 ml 2% aniline sulfate.
Published Version
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