Abstract
The compound N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-5-dimethylammonaphthalene-sulfonamide (DNS-SL(6)) was used as a probe to investigate the egg lecithin – cholesterol – water system. DNS-SL(6) is localized in the polar head group regions of the phospholipid bilayers and undergoes rapid anisotropic motion. Addition of cholesterol has little effect on the mobility or location of the probe but decreases the solubility of the compound in the polar head group region of the bilayers. The partition coefficient of DNS-SL(6) between egg lecithin and buffer is 5.0 × 103 at 22 °C, compared with a partition coefficient of 2.9 × 102 for egg lecithin containing 50 mol % cholesterol. The implications of the effects of cholesterol on membrane structure and passive permeability properties are discussed.
Published Version
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